IDM ¦ Technology/IT
Production of lactoferrin
The use of Radial Flow Column for large-scale
chromatography processes
Author: Michael Feische, Sales Manager Filtration & Separation, Albert Handtmann Armaturenfabrik GmbH & Co. KG,
Arthur-Handtmann-Str. 11, 88400 Biberach, Germany, Phone: +49 7351 342-4532, E-mail: michael.feische@handtmann.de
Lactoferrin (LF) is found in the
milk of all mammalians, also
in milk and whey derived from
cow milk. It is an 80 kDa ironbinding
protein and represents
about 0.3 % of the total protein content
of milk, which equals 60 - 200
mg/l. The thermal stability is 55°C.
Lactoferrin is a very powerful protein:
it has antibacterial, antiviral, antifungal,
anti-tumor, anti-inflammatory
and anti-allergic effects. Isolated from
cow's milk, it is therefore a soughtafter
additive for foods such as functional
food or sports nutrition. The use
of lactoferrin has exploded in recent
years, particularly as a supplement for
baby nutrition.
Column chromatography
In chemistry, chromatography refers to all
those physico-chemical separation processes
in which the separation process is
based on the distribution of a substance
between a mobile and a stationary phase.
Different substances of a sample are retained
to different degrees by the stationary
phase, while the mobile phase takes
over the transport. Chromatographic
analysis methods can include the following:
classical column chromatography,
high-performance liquid chromatography
(HPLC) or ion exchange chromatography
RFC columns for lactoferrin extraction (photo: Handtmann) (IEX) as a special form of HPLC.
34 · November/December 2020 ¦ international-dairy.com
/international-dairy.com
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